Preparation of [15N]- and [4-13C]-Pyridine.
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چکیده
منابع مشابه
Preparation of 13C and 15N labelled RNAs for heteronuclear multi-dimensional NMR studies.
A procedure is described for the efficient preparation of isotopically enriched RNAs of defined sequence. Uniformly labelled nucleotide 5'triphosphates (NTPs) were prepared from E.coli grown on 13C and/or 15N isotopically enriched media. These procedures routinely yield 180 mumoles of labelled NTPs per gram of 13C enriched glucose. The labelled NTPs were then used to synthesize RNA oligomers by...
متن کاملPREPARATION AND ISOMERIZATION OF DICHLOROAMINO-3,5-DIFLUORORO-4- HEPTAFLUOROISOPROPYL-6-METHOXY PYRIDINE
Treatment of 4-heptafluoroisopropyl-2-methoxy-3,5,6-trifluropyridine (4 ) with aqueous ammonia in THF gave 2-amino-3,5-difluoro-4-heptafluoroisopropyl- 6-methoxy pyridine (5) in 7 1% yield. Chlorination of this amine with 'ButOCl at -16°C gave an unstable product, namely 2-dichloroarnino-3,5- difluoro-4-heptafluoroisopropyl-6-methoxy pyridine (6). Iodine-catalysed rearrangement of this dic...
متن کامل[15N,1H]/[13C,1H]-TROSY for simultaneous detection of backbone 15N-1H, aromatic 13C-1H and side-chain 15N-1H2 correlations in large proteins.
This paper describes a [15N,1H]/[13C,1H]-TROSY experiment for the simultaneous acquisition of the heteronuclear chemical shift correlations of backbone amide 15N-1H groups, side chain 15N-1H2 groups and aromatic 13C-1H groups in otherwise highly deuterated proteins. The 15N-1H and 13C-1H correlations are extracted from two subspectra of the same data set, thus preventing possible spectral overl...
متن کامل1H, 13C and 15N resonance assignments of human BASP1
Brain acid-soluble protein 1 (BASP1, CAP-23, NAP-22) appears to be implicated in diverse cellular processes. An N-terminally myristoylated form of BASP1 has been discovered to participate in the regulation of actin cytoskeleton dynamics in neurons, whereas non-myristoylated nuclear BASP1 acts as co-suppressor of the potent transcription regulator WT1 (Wilms' Tumor suppressor protein 1). Here we...
متن کامل1H, 13C, and 15N chemical shift assignments of ZCCHC9
ZCCHC9 is a human nuclear protein with sequence homology to yeast Air1p/Air2p proteins which are RNA-binding subunits of the Trf4/Air2/Mtr4 polyadenylation (TRAMP) complex involved in nuclear RNA quality control and degradation in yeast. The ZCCHC9 protein contains four retroviral-type zinc knuckle motifs. Here, we report the NMR spectral assignment of the zinc knuckle region of ZCCHC9. These d...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1965
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.19-2001